An Oxford team built a RecBCD enzyme assay in three weeks using Synthace automated DOE.

The team needed a biochemical assay for the DNA-repair enzyme RecBCD without slow one-variable-at-a-time optimisation, and had no prior assay experience. Using Synthace they designed and ran two automated DOE experiments covering 10 factors. The page reports a three-week timeline including validation, a z' of 0.59 (n=128), and identified optimal MgCl2:ATP ratios.

Synthace is a software company whose design-of-experiments and lab automation platform helps biology teams develop and transfer assays faster.

This sits in the Design & Stress-Test stage of the AI use-case lifecycle: Named examples from the tools pharma teams use to scope, prototype and benchmark an AI use case before committing to build it — data-catalog, sandbox and evaluation platforms.